Enzymatically Active Self-Standing Protein-Polymer Surfactant Films Prepared by Hierarchical Self-Assembly

Kamendra P. Sharma, Andrew M. Collins, Adam W. Perriman, Stephen Mann

Cross-linked protein-polymer surfactant films consisting of enzymatically active hybrid nanoclusters are prepared using a novel approach based on electrostatically mediated hierarchical self-assembly. The free-standing films are structurally robust, highly hydrophilic, and exhibit sustained fluorescence or recyclable enzymatic phosphatase or oxido-reductase behavior.